Rules of working with a light microscope

1. Put a microscope on your working place.
2. Arrange the objective of a low magnification (x 10) opposite to the object-table opening at the distance 1,5 cm away.
3. Light up the visual field using a mirror.
4. Put a specimen on the object-table with coverslips up. Place the section opposite to microscope frontal lens.
5. Look from the side, lower the microscope tubus down, leave a minimal lumen between the objective and the specimen (up to 1 cm).
6. Look through the objective and slowly rise the tubus up by means of macro-screw until the appearance of the object under observation.
7. Observe the whole specimen while observing it under a low magnification.
8. Choose the most particular portion specimen for studying under a high mag­nification.
9.   Look through the objective of a high magnification (x 40) and raise slowly the tubus by means of microscrew until the appearance of image.
10.   If it's necessary to observe the specimen under the high magnification with objective for immersion oil (x 90) at first you should observe the whole specimen under a low magnification and choose the most particu­lar portion.
11.   Turn a head of gun with objectives and put a drop of immersion oil on the specimen.
12. Arrange the objective of a high magnification (x 90) (the objective will touch a drop of immersion oil).
13. Look through the objective of a high magnification (x 90) and raise slowly the tubus by means of microscrew until the appearance of image.